Ref: FB 340 The ACT-VETube test is an (Activated Clotting Time) in-clinic Screening Test Set checking the efficiency of the intrinsic and common clotting pathway in the dog and cat. Bleeding is a common clinical presentation in Pet Animal Practice caused by vascular injury, trauma, poisoning, surgery, ulcer or tumour. Ranging from mild and self-limiting to severe life-threatening haemorrhage and often seen as spontaneous, multifocal and often, unexpected bleeding requires fast and simple diagnostic and immediate medical attention. Blood clot formation is a complex process. The clotting proteins, or factors, circulate normally in an inactive state as precursors to coagulation. In principle, a series of reactions occurs, in turn acting to catalyse the next reaction, hence the common term “Coagulation Cascade “. The coagulation cascade is divided into extrinsic and intrinsic systems that merge into the common pathway. The intrinsic and common pathways are assessed by either the Activated Clotting Time (ACT) or Activated Partial Thromboplastin Time (APTT). Using a standardized in-vitro coagulation screening test like ACT-VETube enables the veterinarian to discriminate the different haemorrhagic disorders hypothesizing the Activated Thromboplastin Partial Time (APTT) and to treat immediately coagulopathies under practice conditions. This assay detects all coagulopathies except the isolated factor VII deficiency in Beagles and Klee Kai and Alaska malamute. Test Principle Activated Clotting Time (ACT) measures the time in seconds for whole blood to clot using the ACT-VETube at 37C upon exposure to contact activator like diatomaceous earth. Activating the intrinsic cascade diatomaceous earth will coagulate fresh blood, preferably from V.Jugularis, in a definitive time due to coagulation status of the animal. Once the first clot signs form, the test is complete and the required time (=ACT)has to be noted. The Activated Clotting Time (ACT) is defined as the time taken until the appearance of the first unmistakable clots in the blood sample. Measuring the time between the first clot signs and the formation of a solid clot involving the whole sample could give additional information. This period is often increased in dogs with coagulopathy, in normal dogs it is short (seconds).